Journal: Renal Failure

Article Title: Establishment and optimization of a novel mouse model of hyperuricemic nephropathy

doi: 10.1080/0886022X.2024.2427181

Figure Lengend Snippet: Primer sequences for qPCR.

Article Snippet: Anti-E-cadherin and anti-urate transporter 1 (URAT1) antibodies were purchased from Proteintech (Chicago, IL, USA).

Techniques:

Journal: Renal Failure

Article Title: Establishment and optimization of a novel mouse model of hyperuricemic nephropathy

doi: 10.1080/0886022X.2024.2427181

Figure Lengend Snippet: The expression of uric acid reabsorption transporters in HN mice. (A) mRNA levels of Urat1 and Glut9 in HN mice ( n = 8). (B, C) URAT1 protein expression in Western blot analyses ( n = 3). Data are presented as the mean ± SEM. Groups were compared using one-way ANOVA. * indicates a significant difference between the NC group and HN groups: * p < 0.05, ** p < 0.01. Glut9, glucose transporter 9; HN, hyperuricemic nephropathy; Hx, hypoxanthine; NC, normal control; PO, potassium oxonate; Urat1, urate transporter 1.

Article Snippet: Anti-E-cadherin and anti-urate transporter 1 (URAT1) antibodies were purchased from Proteintech (Chicago, IL, USA).

Techniques: Expressing, Western Blot, Control

Journal: Foods

Article Title: Ethanol Extracts from Torreya grandis Seed Have Potential to Reduce Hyperuricemia in Mouse Models by Influencing Purine Metabolism

doi: 10.3390/foods13060840

Figure Lengend Snippet: EST affects transport protein expression in PO-induced hyperuricemia. ( a ) EST treatment effects on protein expression of mouse GLUT9 and NPT1 in isolated liver tissues; ( b ) EST treatment effects on protein expression of mouse URAT1, GLUT9, OCTN2, OAT1, and NPT1 in isolated renal tissues; and ( c ) EST treatment effects on protein expression of mouse NPT1 in isolated intestine tissues. Data are presented as mean ± SEM ( n = 3).

Article Snippet: Antibodies of anti-rabbit glucose transporter 9 (GLUT9), UA anion transporter 1 (URAT1), organic cation transporter 2 (OCTN2), organic anion transporter 1 (OAT1), and urate secretion transporter 1 (NPT1) were purchased from ABclonal Company (Woburn, MA, USA).

Techniques: Expressing, Isolation